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Inhibition efficacy. The relative levels of phosphorylated HER3 for BxPC-3 (A) and DU-145 (B) cells treated with 4 nM heregulin. Signal was measured using Phospho-HER3 ELISA assay and normalized to the signal of the positive control. Cells were treated either with 4 nM of heregulin or 200 nM of affibody construct, followed by stimulation with 4 nM heregulin. For negative control, cells were left untreated. Data are presented as an average ± SD (n = 3).
