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. 2018 Sep 26;7(10):151. doi: 10.3390/cells7100151

Figure 4.

Figure 4

dHUWE1 inhibits JNK signaling and antagonizes the ligand Eiger. (A–C) Activation of the JNK pathway puc69::LacZ-reporter (puc::LacZ, black staining) in control (A), or UAS dHUWE1-RNAi under the control of ptc-Gal4 (B) or hey-Gal4 (C). (D–N) dHUWE1 antagonizes Eiger. Expression of UAS-Eiger results in a small salivary gland (D compared to E), and small eyes (G compared to H). Co-expression of UAS-Eiger with UAS-dHUWE1 suppresses these phenotypes (F,I). (D–F) Indirect immunofluorescence confocal microscopy images of salivary glands derived from wild-type or the indicated transgenic lines. DAPI marks DNA and scale bar is 50 μm. (J–N) Expression of Eiger results in lethality during early larval stages that is partially restored by co-expression of UAS-dHUWE1. (J–M) Representative larval images of the indicated genotypes. (N) Quantification of embryonic hatching at indicated temperatures. Expression of UAS-Eiger together with UAS-GFP (control) is shown in black bars, and expression of UAS-Eiger together with UAS-dHUWE1 is shown in gray bars n = 1000 p = 0.0487 (29 °C, 29); p = 0.0426 (Room temperature, RT).