Table 1.

Oligonucleotide sequences used for polymerase chain reaction in the identification of Staphylococcus aureus and detection of antibiotic resistance genes.

Target Gene	Oligonucleotide Sequence (5′-3′)	Amplicon Size (bp)	PCR Conditions	Reference
nuc	GCG ATT GAT GGT GAT ACG GTT CCA AGC CTT GAC GAA CTA AAG C	255	PCR was performed under the following conditions: One cycle of 95 °C for 5 min, 35 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min, 1 cycle of 72 °C for 10 min.	[33]
mecA	AAA ATC GAT GGT AAA GGT TGG C AGT TCT GCA GTA CCG GAT TTG C	533	Amplification was carried out using 40 cycles of amplification at 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min; this reaction was followed by 5 min of an additional extension at 72 °C.	[6]
blaZ	ACT TCA ACA CCT GCT GCT TTC TGA CCA CTT TTA TCA GCA ACC	173	PCR mixture was subjected to 35 cycles of amplification. The conditions for each cycle were: Denaturation for 1 min at 94 °C, annealing for 1 min at 54 °C, and primer extension for 1 min at 72 °C. Finally, the reaction was incubated at 72 °C for 10 min.	[34]
acc(6)-aph(2)	TTG GGA AGA TGA AGT TTT TAG A CCT TTA CTC CAA TAA TTT GGC T	174	95 °C for 5 min was followed by 30 cycles of denaturation at 95 °C for 60 s, and then annealed at 54 °C for 2 min, and after that extended at 72 °C for 2 min. Finally, an additional extension was achieved for 5 min at 72 °C.	[35]
aph (3’)-1-IIIa	AAA TAC CGC TGC GTA CAT ACT CTT CCG AGC AA	242	95 °C for 5 min was followed by 30 cycles of denaturation at 95 °C for 30 s, and then annealed at 54 °C for 1 min, and after that extended at 72 °C for 2 min. Finally, an additional extension was achieved for 5 min at 72 °C.	[36]
ant (4’)-Ia	AAT CGG TAG AAG CCC AA GCA CCT GCC ATT GCT A	135	95 °C for 3 min was followed by 30 cycles of denaturation at 95 °C for 60 s, and then annealed at 54 °C for 2 min, and after that extended at 72 °C for 2 min. Finally, an additional extension was achieved for 5 min at 72 °C.	[36]
ermA	TAT CTT ATC GTT GAG AAG GGA TT CTA CAC TTG GCT TAG GAT GAA A	139	95 °C for 5 min was followed by 30 cycles of denaturation at 95 °C for 30 s, and then annealed at 54 °C for 1 min, and after that extended at 72 °C for 2 min. Finally, an additional extension was achieved for 5 min at 72 °C.	[35]
ermB	CTA TCT GAT TGT TGA AGA AGG ATT GTT TAC TCT TGG TTT AGG ATG AAA	142	95 °C for 5 min was followed by 30 cycles of denaturation at 95 °C for 30 s, and then annealed at 54 °C for 1 min, and after that extended at 72 °C for 2 min. Finally, an additional extension was achieved for 5 min at 72 °C.	[37]
ermC	CTT GTT GAT CAC GAT AAT TTC C ATC TTT TAG CAA ACC CGT ATT C	190	95 °C for 5 min was followed by 30 cycles of denaturation at 95 °C for 30 s, and then annealed at 54 °C for 1 min, and after that extended at 72 °C for 2 min. Finally, an additional extension was achieved for 5 min at 72 °C.	[35]
msrA	TCC AAT CAT TGC ACA AAA TC AAT TCC CTC TAT TTG GTG GT	163	94 °C for 5 min, 30 cycles of 94 °C for 45 s, 50 °C for 60 s, 72 °C for 60 s, final extension at 72 °C for 10 min.	[35]
tetK	GTA GCG ACA ATA GGT AAT AGT GTA GTG ACA ATA AAC CTC CTA	360	Initial denaturation at 94 °C for 3 min, 25 cycles of 94 °C for 1 min, annealing at 55 °C for 1 min, and extension at 72 °C for 1 min. The final extension was carried out at 72 °C for 10 min.	[37]
tetM	AGT GGA GCG ATT ACA GAA CAT ATG TCC TGG CGT GTC TA	158	Initial denaturation at 94 °C for 3 min, 25 cycles of 94 °C for 1 min, annealing at 55 °C for 1 min, and extension at 72 °C for 1 min. The final extension was carried out at 72 °C for 10 min.	[37]