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. 2018 Sep 12;59(11):2237–2252. doi: 10.1194/jlr.D089136

TABLE 3.

Product distribution of epoxidation of DHA by BM3 enzymes on large scale

Enzyme % Recovered DHA % 2a + 2ba Ratio (3a:3b)b % 2ca % Major Enantiomer (2a)c % Major Enantiomer (2b)c
WT-BM3 24 47 80:20 22 >99 >99
BM3 (F87V) 33 36 61:39 22 89 >99
a

Yields are based on % of theoretical for a given product.

b

Ratio determined after esterification and separation of regioisomers.

c

As determined by chiral HPLC (see Materials and Methods and Figs. 3, 4).