Fig. 2.
Fasting-induced hepatosteatosis is exacerbated by PPARA ablation. WT (Ppara+/+), full-body Ppara-null (Ppara−/−), and hepatocyte-specific Ppara-null (PparaΔHep) mice fed al libitum or fasted for 24 h. Gross liver physiology (A) and liver TG levels (B) after 24 h fast. H&E staining of sections from fed (C) and fasted (D) mouse livers. ORO staining (E) of fasted liver sections shows differential lipid accumulation. Data are mean ± SD (n = 5). Asterisks above bars indicate significance versus fed group of the same genotype. Representative images (n = 5) taken at 40× (scale bars = 500 mm) and 100× magnification (scale bars = 100 mm). *** P < 0.001.