Fig. 7.
FAO is elevated in extrahepatic tissues of fasted PparaΔHep mice. Mice were fasted for 24 h, and then tissues collected for RNA isolation and qRT-PCR analysis. Expression of PPARA target gene mRNAs involved in β-oxidation (Acadm, Cpt1b, and Ehhadh) and fasting response (Fgf21 and Pdk4) were measured in BAT (A), heart (B), liver (C), and skeletal muscle (D). Data normalized to Actb and expressed as fold change WT control. Data are mean ± SD (n = 5). Asterisks above bars indicate significance versus fed group of the same genotype. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant.