Fig. 4.
Lipoprotein profiles of ApoEshl and ApoEshl/GM3S−/− mice. Lipoprotein-associated cholesterol levels in 16- to 18-week-old male mice were determined by HPLC. A, B: Representative HPLC patterns of (A) ApoEshl and (B) ApoEshl/GM3S−/− serum. A 5 μl serum sample was injected onto two tandem gel permeation columns and eluted with TSK eluent LP-1 at a flow rate of 0.7 ml/min. Pink lines represent cholesterol, and blue lines represent triglyceride. Serum total cholesterol and total triglyceride levels are 587 ± 65 and 59 ± 33 mg/dl (A) and 259 ± 75 and 35 ± 11 mg/dl (B), respectively. Lipoprotein subclasses determined from observed elution times are presented. C–F: Chylomicron, VLDL, LDL, and HDL, respectively (n = 3 per group). *P < 0.05 and **P < 0.01.