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. 2018 Sep 20;9(10):464. doi: 10.3390/genes9100464

Figure 1.

Figure 1

Hybridization of mixed Cot-1 DNA from liver, ovary and testis on metaphase chromosomes. (A) A DAPI-stained metaphase spread. (B) Asian seabass Cot-1 DNA fraction hybridization (green). Chromosomes were contrasted by 4′,6-diamidino-2-phenylindole (DAPI; blue). Empty arrowheads indicate B chromosomes (Bchrs). White arrowheads indicate Bchrs with a low level of Cot-1 signal. The bar is 5 μm. The inset shows magnified examples of Bchrs with a low (left) and high (right) intensity of Cot-1 signal; the bar for the framed B chromosomes is 1 µm.