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. 2018 Sep 20;9(10):464. doi: 10.3390/genes9100464

Figure 4.

Figure 4

Population-scale variation in the copy number of B chromosome-associated regions in the Asian seabass species complex. (A) Geographic regions across the native range of Asian seabass: Western and Eastern Coast of India; South-East Asia and the Philippines, Australia/Papua New Guinea. (B) Distribution of inferred genome fragment copy number in 1 kb windows across the Asian seabass genome: Homozygous deletion, 0.1–1.4; heterozygous deletion, 1.5–2.5; normal diploid copy number, 2.6–4; heterozygous duplication, > 4; multiple duplications. X-axis: DNA copies; Y-axis: Number of 1 kb windows. (C) Population-scale variations in the copy number of the Bchr6-associated region and control genome fragment were demonstrated for the three geographical regions. X-axis: Number of genomic fragments which correspond to a particular 1 kb window of the reference genome for each individual. Y-axis: Genomic fragment copy number in 1 kb windows across the Bchr6-associated region and non-repeat genomic fragment. The order is indicated in Table S3: Indian region (blue), South-East Asia and Philippine region (orange), Australia/Papua New Guinea region (green). The copy number of control genes (gal3st3, znfx-1) is presented in 442–452 regions. The estimation of copy number variation was performed after repeat masking, so the number of DNA fragments and size of the analyzed sequences were less than the source. The Asian seabass genome assembly version 2.0 was used as a reference genome.