Figure 4. WNT7 Expression Strongly Correlates with OPC Markers in Human Glioma.

(A and B) Pearson correlation coefficients of OLIG2 and NKX2–2 with WNT7B mRNA levels in normal brain, glioblastoma (GBM), grade III oligodendroglioma (OD Gr.III) (A) and in GBM subtypes: classical (C), mesenchymal (M), neural (N), and proneural (PN) (B). n is indicated for each group (***p < 0.001).

(C) Pearson correlation coefficients of OLIG2 with WNT7B mRNA levels in p53 WT and mutant grade III OD (OD). *p < 0.05.

(D–F) Immunostaining for Idh1-R132 (D), Wnt7a, vWF, Olig2 (E) and Idh1-R132, Wnt7a, Wnt7b, Olig2 (F) on IDH1^R132H-mutant grade III OD. Black arrowheads indicate tumor cells (n = 4 cases). Scale bars, 20 μm (D and E) and 10 μm (F).

(G and H) Immunostaining for Olig2, Wnt7b, collagen IV (G) and Wnt7a, Olig2, CD31 (H) on proneural glioblastoma. Yellow arrowheads indicate Olig2⁺ Wnt7a⁺ cells (n = 3 cases). Scale bars, 50 μm (H, left) and 10 μm (G), inset in (H).

(I) Immunostaining for CD31, Lef1, Olig2, and collagen IV on IDH1^R132H-mutant grade III OD. White arrowheads indicate Lef1⁺ cells (n = 3 cases). Scale bars, 50 μm (left) and 20 μm (right). (J) Immunostaining for CD31 and Lef1 on proneural glioblastoma. White arrowheads indicate Lef1⁺ cells (n = 3 cases). Scale bar, 20 μm. (K) Quantifications of the percentage of Lef1⁺ CD31⁺ cells in grade III OD (OD-III) and proneural glioblastoma (PN GBM). Data are means ± SEM (n = 3 cases per group). See also Figure S3 and Table S3