REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-b actin	Sigma-Aldrich	Cat# A5316; RRID: AB_476743
Mouse monoclonal anti-b catenin	Millipore	Cat# 05-665; RRID: AB_309887
Mouse monoclonal anti-alpha tubulin	Sigma-Aldrich	Cat# T5168; RRID: AB_477579
Mouse monoclonal anti-b tubulin	Sigma-Aldrich	Cat# T4026; RRID: AB_477577
Rabbit polyclonal anti-CD31	Abcam	Cat# ab28365; RRID: AB_726365
Rat monoclonal anti-CD31	BD Biosciences	Cat# 550274; RRID: AB_393571
Goat polyclonal anti-Collagen IV	Millipore	Cat# ab769; RRID: AB_92262
Mouse monoclonal anti-human EGFR	Dako	Cat# M7298; RRID: AB_2286187
Sheep polyclonal anti-fibrinogen	Abcam	Cat# ab118533; RRID: AB_10900171
Mouse monoclonal anti-GADPH	Abcam	Cat# ab8245; RRID: AB_2107448
Chicken polyclonal anti-GFP	Aves Lab	GFP-1020; RRID: AB_10000240
Rabbit polyclonal anti-GFAP	Dako	Cat# Z0334; RRID: AB_10013382
Rabbit polyclonal anti-Histone H3 (acetyl K27)	Abcam	Cat# ab4729; RRID: AB_2118291
Mouse monoclonal anti-HLA Class 1 ABC	Abcam	Cat# ab70328; RRID: AB_1269092
Rabbit polyclonal anti-Iba1	Wako	Cat# 019-19741; RRID: AB_839504
Mouse monoclonal anti-Idh1-R132H	Dianova	Cat# DIA-H09; RRID: AB_2335716
Rabbit polyclonal anti-Ki67	Thermo Scientific	Cat# RM-9106; RRID: AB_2341197
Rabbit monoclonal anti-Lef1	Cell Signaling	Cat# 2230; RRID: AB_823558
Rabbit polyclonal anti-LYVE1	Novus Biologicals	Cat# NB100-725; RRID: AB_10003273
Rabbit polyclonal anti-NG2	Millipore	Cat# AB5320; RRID: AB_91789
Mouse monoclonal anti-Nkx2.2	DSHB	Cat# 74.5A5; RRID: AB_531794
Mouse monoclonal anti-Olig2	Charles D. Stiles, Harvard Medical School, Cambridge, Massachusetts	RRID: AB_10807410
Rabbit polyclonal anti-Olig2	Millipore	Cat# AB9610; RRID: AB_570666
Rabbit polyclonal anti-Olig2	Charles D. Stiles, Harvard Medical School, Cambridge, Massachusetts	DF308; RRID: AB_2336877
Rabbit polyclonal anti-p53	Santa Cruz	Cat# FL-393; RRID: AB_653753
Rat monoclonal anti-CD140a	BD Biosciences	Cat# 558774; RRID: AB_397117
Rabbit monoclonal anti-Tcf4	Cell Signaling	Cat# 2569S; RRID: AB_2199816
Mouse monoclonal anti-VEGF	Thermo Fisher	Cat# MS-1467; RRID: AB_144688
Rabbit polyclonal anti-vWF	Abcam	Cat# ab6994; RRID: AB_305689
Goat polyclonal anti-Wnt7a	Santa Cruz	Cat# sc-26361; RRID: AB_2215743
Rabbit polyclonal anti-Wnt7a	Abcam	Cat# ab100792; RRID: AB_10858110
Rabbit polyclonal anti-Wnt7b	Abcam	Cat# ab94915; RRID: AB_10675749
Bacterial and Virus Strains
eGFP adenovirus	Vector Biolabs	Cat# 1060
GFP-GLuc lentivirus	Wurdinger et al., 2008
mCherry lentivirus	Massachusetts General Hospital Viral Vector Core Facility
Biological Samples
Human grade III oligodendroglioma and proneural GBMs	The Brain Tumor SPORE Biospecimen/Pathology Core, University of California San Francisco	http://cancer.ucsf.edu/research/spores/brain-spore/cores#one
Paired pre-Bev (biopsies) and post-Bev (autopsies) GBMs	Tissue Bank Core, Mass General Hospital
Human primary GBMs flash-frozen tissue	Tissue Bank Core, Mass General Hospital
Chemicals, Peptides, and Recombinant Proteins
XAV-939	Tocris Bioscience	Cat# 3748; CAS: 284028-89-3
LGK-974	Selleckchem	Cat# S7143; CAS: 1243244-14-5
B20-4.1	Genentech/Hoffmann La-Roche
Bevacizumab	Genentech	CAS: 216974-75-3
Temolozomide	Tocris Bioscience	CAS: 85622-93-1
Deposited Data
Raw and analyzed data	This paper	GEO: GSE110052
TCGA data portal	TCGA	https://tcga-data.nci.nih.gov/tcga/
Allen Brain Institute data portal	ABI	http://human.brain-map.org/static/download
Experimental Models: Cell Lines
Human: MGG8 cells	Wakimoto et al., 2012
Human: MGG6 cells	Wakimoto et al., 2012
Human: D-54 cells	Bigner et al., 1981	RRID:CVCL_7185
Human: U-87MG ATCC cells	ATCC	Cat# 300367/p658_U-87_MG, RRID:CVCL_0022
Human: SF10417 cells	Joseph Costello, University of California San Francisco
Experimental Models: Organisms/Strains
Mouse: Olig2tm2(TVA,cre)Rth/J	The Jackson Laboratory	RRID:IMSR_JAX:011103
Mouse: FVB.129-Trp53tm1Brn/Nci	NCI	RRID:IMSR_NCIMR:01XC2
Mouse: B6;129S1-Wnt7atm1Amc/J	The Jackson Laboratory	RRID:IMSR_JAX:004715
Mouse: B6;129X1-Wnt7btm2Amc/J	The Jackson Laboratory	RRID:IMSR_JAX:008467
Mouse: B6.129-Cdkn2atm1Rdp/Nci	NCI	RRID:IMSR_NCIMR:01XB1
Mouse: TieP-GFP Rag1−/−	Cheng et al., 2011
Mouse: IcrTac:ICR-Prkdcscid	Taconic	RRID:IMSR_TAC:icrsc
Mouse: NOD.CB17-Prkdcscid/J	The Jackson Laboratory	RRID:IMSR_JAX:001303
FVB/NCrl	Charles River	RRID:IMSR_CRL:207
Oligonucleotides
Primers for mouse Lef1, LYVE-1, Plvap, Slc2a1, Prox1, Tbx1, VEGFR1/2/3, Wnt5a, Wnt7a/b, Vegfa/b/c, see Table S5	This paper	N/A
Primers for mouse Lef1, LYVE-1, Plvap, Slc2a1, Prox1, Tbx1, VEGFR1/2/3, Wnt5a, Wnt7a/b, Vegfa/b/c, see Table S5	This paper	N/A
Software and Algorithms
R package		http://cran.us.r-project.org
Gene Cluster 3.0	de Hoon et al., 2004	RRID:SCR_002250
Imaris v7.6-8.2	Bitplane	RRID:SCR_007370
Java Treeview	Saldanha, 2004	RRID:SCR_013503