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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: J Immunol. 2018 Sep 7;201(8):2244–2255. doi: 10.4049/jimmunol.1800110

Figure 3. Impact of Casp8 and RIPK3 deficiency on DC activation following MCMV infection.

Figure 3.

(A and B) Representative contour plots showing frequencies of mature cDCs (A) and bar graphs showing total mean numbers ± S.E.M. of cDC1s and cDC2s (B) in spleens of infected mice. (C and D) Representative histograms showing CD80 and CD86 expression on cDC1s (C) and cDC2s (D) from either mock or infected mice. (E and F) Bar graphs showing concentrations ± S.E.M. of IL-12(p70) in spleen homogenate (E) and serum (F) as quantified by ELISA. Dash lines indicate limit of detection. Eight-week-old WT, HET and DKO mice were inoculated intraperitoneally (i.p.) with 105 PFU MCMV (V70 strain) per mouse. Splenocytes were analyzed at 1.5 dpi by flow cytometry, as described in Figure 1. Spleen homogenates or sera were analyzed by ELISA. Combined data of n = 11 mice from up to three independent experiments are shown. *, p < 0.05; **, p < 0.01; ***, p < 0.001.