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Effect of magnesium (A), manganese (B) and cobalt (C) on the activity of CaPho15p (solid line) and CpPho15p (dashed line). The measurements were performed in triplicate, using immobilised phosphatases and pNPP as a substrate. The procedure is described in the Materials and Methods section. Comparison of CaPho15p (dark bars) and CpPho15p (light bars) activities in the presence of different divalent cations (D). For this comparison, 10 mM concentration of each cation was used.
