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K m graphs of CaPho15p (A, B) and CpPho15p (C, D) for 2-P-Glycolate (A, C) and 2-Glycerolphosphate (B, D). The Km and Vmax values are shown in Table 1. The measurements were performed in triplicate, using immobilised phosphatases and corresponding substrate. The procedure is described in the Materials and Methods section.
