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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Nat Protoc. 2018 Jul;13(7):1632–1661. doi: 10.1038/s41596-018-0006-9

Table 1 |.

Pooling fractions for proteome analysis

1 2 3 4 5 6 7 8 9 10 11 12
A A A A A A A A A A A
B 10 9 8 7 6 5 4 3 2 1 A A
C 11 12 13 14 15 16 17 18 19 20 21 22
D 10 9 8 7 6 5 4 3 2 1 24 23
E 11 12 13 14 15 16 17 18 19 20 21 22
F 10 9 8 7 6 5 4 3 2 1 24 23
G 11 12 13 14 15 16 17 18 19 20 21 22
H 24 24 24 24 24 24 23

Combining strategy for a 4.6-mm column. Underlined numbers indicate the fractions meant to be pooled into fraction 1. Italicized numbers indicate the fractions meant to be pooled into fraction 2. These indications are meant to make pooling easier visually.