| Parameter | Value |
|---|---|
| Variable modification | Oxidation (M) |
| Acetyl (protein N-term) | |
| Deamidation (N) | |
| PyroGlu (Q) | |
| Fixed modification | Carbamidomethyl (C) |
| TMT-10 (peptide N-term, K) | |
| Digest | Trypsin Allow P |
| Maximum missed cleavages | 4 |
| Maximum charge | 5 |
| Precursor mass tolerance (p.p.m.) | 20 |
| Product mass tolerance (p.p.m.) | 20 |
| Peptide FDR (%) | 1 |
| Protein FDR (%) | 0 |
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