| Time interval (min) | Gradient (%B) | Flow rate (nl/min) |
|---|---|---|
| 0 | 2 | 200 |
| 1 | 6 | 200 |
| 85 | 30 | 200 |
| 94 | 60 | 200 |
| 95 | 90 | 200 |
| 100 | 90 | 200 |
| 101 | 50 | 500 |
| 110 | 50 | 500 |
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