Parameter	Value
Polarity	Positive
Full MS
Microscans	1
Orbitrap resolution	60,000
AGC Automatic Gain Control target	4 × 105 ion counts
Maximum ion time	50 ms
Scan range	350–1,800 m/z
dd-MS2
Microscans	1
Detector type	Orbitrap
Orbitrap resolution	50,000 (TMT-10)
AGC target	1 × 105 ion counts
Maximum ion time	105 ms
Cycle time: time between master scans	2 s
Isolation window	0.7 m/z
Fixed first mass	110 m/z
Activation type	Higher-energy collision dissociation (HCD)
HCD energy (%) dd settings	38 (TMT)
Monoisotopic peak determination	Peptide
Charge exclusion	Include 2–6 charge states
Exclude isotopes	On
Dynamic exclusion	20 s