Figure 1.

Schematic representation of the methods used for cell isolation and culture. All assays were run in isolated cells from 20 cows (10 early- and 10 mid-lactation cows), were performed in duplicate wells (technical replicate) and individually analyzed for phagocytosis, oxidative burst, and proliferation. For mRNA abundance and TNF-α in spent media, cells, and media from two cultured wells were pooled.