Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The amount and order of each reactant of α-glucosidase inhibition test.
| Reagents | Volume (μL) |
|||
|---|---|---|---|---|
| Blank group | Control group | Sample blank group | Sample group | |
| PBS | 20 | 10 | 20 | 10 |
| Compounds/inhibitors | 0 | 0 | 10 | 10 |
| PNPG | 20 | 20 | 20 | 20 |
| Water | 10 | 10 | 0 | 0 |
| Mix well and incubate at 37 °C for ten minutes | ||||
| α-Glucosidase | 0 | 10 | 0 | 10 |
| Mix well and react at 37 °C for 20 minutes | ||||
| Na2CO3 | 70 | 70 | 70 | 70 |
