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. 2018 Oct 23;9:2437. doi: 10.3389/fimmu.2018.02437

Figure 7.

Figure 7

The function of SLP, f PGN and LTA in regulating the expression of CD207 on THP-1. (A) The structure of L. crispatus and L. fermentum were observed by transmission electron microscopy (TEM). L. crispatus and L. fermentum untreated and treated with huaridine hydrochloride were observed by TEM with high magnification. (B) Detection of SLPs from L. crispatus and L. fermentum by SDS-PAGE. (C) Identified SLPs from L. crispatus and L. fermentum by WB. The target protein is about 55 kDa. (D) CD207 expression induced by SLP from L. crispatus. Cells were treated with 1 mg/ml SLP from L. crispatus and L. fermentum. (E) CD207 expression induced by PGN and LTA from L. crispatus and L. fermentum. Cells were treated with 1 mg/ml PGN or LTA from L. crispatus and L. fermentum. (F) CD207 expression induced by PGNs from Bacillus subtilis, E. coli JM109 and E. coli DH5α. Cells were treated with 1 mg/ml PGN o from Bacillus subtilis, E. coli JM109, and E. coli DH5α.