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. 2018 Nov 1;18:1058. doi: 10.1186/s12885-018-4952-y

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Schematic representation of the workflow. Small-EV proteins from HT-29 and HCT-116 colon cancer cell lines were identified by proteomic analysis and candidate proteins predicted to be upregulated in colon cancer patients compared to the healthy controls were selected. Candidate proteins were verified by western blot analysis. Among these candidates, TSPAN1 was further validated by ELISA