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. 2018 Nov 1;18:1058. doi: 10.1186/s12885-018-4952-y

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characterization of small-EVs isolated from HT-29 and HCT-116 colon cancer cells. a Viability of HT-29 and HCT-116 colon cancer cells following exposure to serum-free medium was measured using the MTT assay. b Small-EVs (10 μg protein) from HT-29 and HCT-116 cells were subjected to immunoblotting with antibodies against small-EV marker proteins, namely CD63 and Alix. c, d Small-EVs derived from HT-29 and HCT-116 cells were analyzed by transmission electron microscopy. All small-EV samples contained vesicles of variable sizes in the range of 70–200 nm. Scale bar, 100 nm. e, f Size distribution of small-EVs derived from two different colon cancer cell lines was measured using nanoparticle tracking analysis. The images shown are representative and the size of HT-29- and HCT-116-derived small-EVs is 184.2 ± 46.5 and 178.5 ± 44.4 nm, respectively (n = 3)