Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 1;18:265. doi: 10.1186/s12870-018-1494-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 9 — qRT-PCR analysis of VcRR2 in Mu-Legacy and Mu-Legacy-T₁ using E1F and E1R primers. Eukaryotic translation initiation factor 3 subunit H is the internal control. Log₂(fold-change) in Legacy-VcDDF1-OX was calculated by -∆∆Ct = −[(Ct_GOI – Ct_nom)_{Mu-Legacy or Mu-Legacy-T1} – (Ct_GOI – Ct_nom)_Legacy]. Average fold-changes ± standard deviation of three biological replicates for each of Mu-Legacy, Mu-Legacy-T₁, and nontransgenic ‘Legacy’ plants were plotted. Significant average fold-change determined using a Student’s t-test is denoted. Asterisks (**) indicate p < 0.01. The fold change of RNA-seq is an average of the Log₂Fold change (Mu-Legacy/Legacy) for three DE isoforms listed in Table 1