Fig. 3.
Interaction plot of mean scratching scores of mice reared for 6 weeks on the antibiotic (AB) vancomycin or on tap water (control) and then treated with either distilled water (H2O), TNF-α, or TNF-α and peanut butter (TNF-α + PB) (n = 6–9/group). Two week later mice were then challenged with the peanut antigen. Scratching behaviour was observed for 60 s 15 min following the ear punch challenge on a scale of 0–2, with 3 being the most severe scratching response. *p < 0.05 by 2 × 3 ANOVA and Fischer’s least significant difference post hoc tests. Antibiotic treatment significantly affected ear-scratching responses and showed a significant interaction with sensitization treatment effects (p < 0.05)