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. 2018 Nov 1;11:572. doi: 10.1186/s13071-018-3143-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Linear correlation between the log of DNA concentration and Ct values. The qPCR-MEint curve was obtained amplifying serial dilutions of genomic DNA (from 2 to 2 × 10^-5 ng/reaction tube) purified from L. infantum MHOM/FR/78/LEM75 (y = -3.59x + 20.58). Each reaction was performed in triplicate. Reaction efficiency = 90%, R² = 0.996