Fig. 3.

C19 induces apoptosis in colorectal cancer cells. a Cells were treated with the IC₅₀ of C19 for 24, 48 and 72 h and cell lysates were analyzed for cleaved PARP. β-actin served as loading control. Representative blots of three independent experiments are shown. b Densitometric analysis of c-PARP normalized to total PARP levels in HCT116 and LoVo cells treated with C19 for 72 h. Error bars represent SEM. *p < 0.01 (c) Annexin V and PI analysis of HCT116 and LoVo cells treated with the IC₅₀ concentration of C19 or vehicle alone for 72 h. C19 treatment strongly increased the percentages of LoVo cancer cells in early (Annexin V+ PI-) and late apoptosis (Annexin V+ PI+)