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. 2018 Nov 1;18:264. doi: 10.1186/s12870-018-1489-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Darkness-induced leaf senescence and gene expression analysis. a Detached flag leaves from IR64 and spl21 at the heading stage were incubated with normal light (H₂O) and darkness (H₂O) at 30 °C for 5 d. Scale bar = 1 cm. b Expression profile of senescence genes in IR64 and spl21 at heading stage. c Expression profile of photosynthesis-related genes in IR64 and spl21 at heading stage; the expression level of each gene in IR64 was normalized to 1. Error bars in (b, c) indicate standard deviations of three independent samples. Data are means ± SD of three biological replicates. Single asterisk denotes P ≤ 0.05, and double asterisks denote P ≤ 0.01 (Student’s t-test)