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. 2018 Oct 31;5(Pt 6):866–879. doi: 10.1107/S2052252518013854

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© Woo-Chang Chung et al. 2018

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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Molecular interactions of vPIP mutants with PARP-1 or RTA. (a) Interaction between vPIP mutants and PARP-1. MYC-tagged vPIP mutants were co-transfected with FLAG-tagged PARP-1 into HEK293T cells. The cells were harvested 48 h post-transfection and analyzed by co-IP assays with the anti-FLAG antibody. The results were examined by Western blotting. The band intensity of MYC in IP blots was measured using the ImageJ software. (b) Inhibition of interactions between RTA and PARP-1 by vPIP mutants. MYC-tagged vPIP mutants were co-transfected with FLAG-tagged RTA into HEK293T cells. The cells were harvested 48 h post-transfection and subjected to co-IP assays with the anti-FLAG antibody. The results were examined by Western blotting. The band intensity of PARP-1 and MYC in IP blots was measured in ImageJ. (c) A summary of subcellular localization, protein–protein interactions and functional activities of WT vPIP and mutants.