REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD117 APC	BD Biosciences	Cat#553356
Ly-6A/E APC-Cy7	BD Biosciences	Cat#560654
CD34 FITC	BD Biosciences	Cat#553733
CD16/CD32 PE-Cy	eBiosciences	Cat#25-0161
Ly-6G and Ly-6C Biotin	BD Biosciences	Cat#553124
Cd11b Biotin	BD Biosciences	Cat#553309
CD45R/B220 Biotin	BD Biosciences	Cat#553086
CD3e Biotin	eBiosciences	Cat#13-0037-82
Ter119 Biotin	BD Biosciences	Cat#553672
Streptavidin eFluor® 450	eBiosciences	Cat#48-4317-82
CD11b PE	BD Biosciences	Cat#555388
CD15 APC	BD Biosciences	Cat#561716
CD117 APC	BD Biosciences	Cat#553356
RUNX1 polyclonal	Abcam	Cat#ab23980
H3K27ac polyclonal	Abcam	Cat#ab4729
H3K4me1 polyclonal	Abcam	Cat#ab8895
H3k27me3 monoclonal	Abcam	Cat#ab6002
BRG1 monoclonal	EPITOMICS	Cat#2822-1
RING1B polyclonal	Abcam	Cat#ab3832
MYC polyclonal	Santa Cruz	Cat#N-262
p300 polyclonal	BETHYL	Cat#A300-358A
GAPDH monoclonal	Cell Signaling	Cat#3683
Biological Samples
human AML samples	UPENN	N/A
human AML samples	University Hospital Heidelberg	N/A
human cord blood	UMASS	N/A
Chemicals, Peptides, and Recombinant Proteins
AI-10-49	John Bushweller	N/A
JQ1	James Bradner	N/A
JQ1	ApexBio	Cat#A1910
PRT 4165	Tocris Bioscience	Cat#5047
pIpC	GE healthcare	Cat#27-4732-01
4-Hydroxytamoxifen	Sigma	Cat#H7904
Recombinant Human IL-3	Peprotech	Cat#200-03
Recombinant Human IL-6	Peprotech	Cat#200-06
Recombinant Human FLT3L	Peprotech	Cat#300-19
Recombinant Human SCF	Peprotech	Cat#300-07
Recombinant Human TPO	Peprotech	Cat#300-18
Recombinant Murine IL-3	Peprotech	Cat#213-13
Recombinant Murine IL-6	Peprotech	Cat#216-16
Recombinant Murine SCF	Peprotech	Cat#250-03
Critical Commercial Assays
PureLink® RNA Mini Kit	Life Technologies	Cat#12183018A
SUPERSCRIPT III	Thermo Fisher Scientific	Cat#18080-044
Power SYBR® Green PCR Master Mix	Applied Biosystems	Cat#4367659
Annexin V Apoptosis Detection Kit	BD Biosciences	Cat#559763
NE-PER™ Nuclear and Cytoplasmic Extraction Reagents	Thermo Fisher Scientific	Cat#78833
CellTiter 96® AQueous One Solution Cell Proliferation Assay	Promega	Cat#G3580
True seq Nano DNA LT kit	Illumina	Cat#15041757
Nextera DNA Sample Preparation Kit	Illumina	Cat#FC-121-1030
KAPA Genotyping kit	Kapabiosystems	Cat#KK7352
TruSeq RNA library kit	Illumina	Cat#RS-122-2001
CD34 MicroBead Kit	Milteny Biotec	Cat#130-046-702
EasySep® Mouse Hematopoietic Progenitor Cell Enrichment Kit	Stemcell Technologies	Cat#19756
Amaxa® Cell Line Nucleofector® Kit V	Lonza	Cat#VCA-1003
Deposited Data
SuperSeries of RNA-seq, ChIP-seq, ATAC-seq and 5C	This paper	GEO: GSE101791
RNA-seq in ME-1 cells	This paper	GEO: GSE101788
ChIP-seq in ME-1 cells	This paper	GEO: GSE101789
ATAC-seq in ME-1 cells	This paper	GEO: GSE101790
5C in ME-1 cells	This paper	GEO: GSE109764
ChIP-seq in ME-1 cells	Mandolli et al., 2014	GEO: GSE46044
ChIP-seq in K562 cells	Pugacheva et al., 2015	GEO: GSE70764
Experimental Models: Cell Lines
Human: ME-1 cells	DSMZ	Cat#ACC 537
Human: U937 cells	ATCC	Cat#CRL-1593.2
Human: K562 cells	ATCC	Cat#CCL-243
Human: Jurkat cells	DSMZ	Cat#ACC-282
Human: Kasumi-1 cells	ATCC	Cat#CRL-2724
Human: THP-1 cells	ATCC	Cat#TIB-202
Human: NB4 cells	DSMZ	Cat#ACC-207
Human: MV4:11 cells	ATCC	Cat#CRL-9591
Human: 293T/17 cells	ATCC	Cat#CRL-11268
Experimental Models: Organisms/Strains
C57BL/6J	Taconic Biosciences	N/A
Oligonucleotides
ChIP-qPCR Primers (See Table S2)	This paper	N/A
RT-PCR Primer (See Table S2)	This paper	N/A
sgRNA (See Table S2)	This paper	N/A
CUT&RUN Primer (See Table S2)	This paper	N/A
ATAC-qPCR Primer (See Table S2)	This paper	N/A
shRNA (See Table S2)	This paper	N/A
Recombinant DNA
Plasmid: shRNA MYC4	UMMS RNAi Core Facility	Cat#TRCN0000174055
Plasmid: shRNA MYC6	UMMS RNAi Core Facility	Cat#TRCN0000010390
Plasmid: pLKO.1 puro	Sigma	Cat#SHC002
Plasmid: shRNA Myc1 (Myc1891) CTCGAGAAGGTATATTGCTGTTGACA GTGAGCGCACGACGAGAACAGTTG AAACATAGTGAAGCCACAGATGTAT GTTTCAACTGTTCTCGTCGTTT GCCTACTGCCTCGGAATTC	Zuber et al., 2011b	N/A
Plasmid: shRNA-Myc2 (Myc2105) CTCGAGAAGGTATATTGCTG TTGACAGTGAGCGCCTGCC TCAAACTTAAATAGTATAGTGAAGCCA CAGATGTATACTATTTAAGTTTGA GGCAGTTGCCTACTGCCTCGGAATTC	Zuber et al., 2011b	N/A
Plasmid: shRNARen (Ren713) TGCTGTTGACAGTGAGCGCAGGAA TTATAATGCTTATCTATAG TGAAGCCACAGATGTATAGATA AGCATTATAATTCCTAT GCCTACTGCCTCGGA	Zuber et al., 2011b	N/A
Plasmid: pLKO. 1 - TRC cloning vector	Moffat et al., 2006	Addgene#10878
Plasmid: psPAX2	Trono Lab Packaging and Envelope Plasmids (unpublished)	Addgene#12260
Plasmid: pMD2.G	Trono Lab Packaging and Envelope Plasmids (unpublished)	Addgene#12259
Plasmid: pLentiCRISPRv2	Sanjana et al., 2014	Addgene#52961
Plasmid: pDecko-mCherry	Pulido-Quetglas et al., 2017	Addgene#78534
Plasmid: MYC-ER	Ricci et al., 2004	Addgene#19128
Software and Algorithms
Bioconductor v3.4		http://www.bioconductor.org
ChIPpeakAnno v3.9	Zhu, 2013; Zhu et al., 2010	https://bioconductor.org/packages/release/bioc/html/ChIPpeakAnno.html
ATACseqQC v1.0.3	Bioconductor package	https://bioconductor.org/packages/release/bioc/html/ATACseqQC.html
Bowtie2 v2.1.0	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
MACS2 v2.1.0	Zhang et al., 2008	https://github.com/taoliu/MACS
FastQC v0.10.1	Babraham Bioinformatics	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
Tophat v2.0.9	Trapnell et al., 2009	https://ccb.jhu.edu/software/tophat/index.shtml
Cufflinks v2.2.0	Trapnell et al., 2012	http://cole-trapnell-lab.github.io/cufflinks/
Diffbind v2.4.8	Ross-Innes et al., 2012	https://bioconductor.org/packages/release/bioc/html/DiffBind.html
Picard tools 1.96	Broad Institute	https://broadinstitute.github.io/picard/
Bedtools v1.25.0	Quinland et al., 2010	http://bedtools.readthedocs.io/en/latest/
Fastx-toolkit v.0.0.18	Hannon Laboratory	http://hannonlab.cshl.edu/fastxtoolkit/
GSEA	Broad Institute	http://software.broadinstitute.org/gsea/index.jsp
Molecular Signatures Database v6.0	Broad Institute	http://software.broadinstitute.org/gsea/msigdb/index.jsp
GraphPad Prism 6.0	GraphPad	https://www.graphpad.com
FlowJo Software	FlowJo LLC	https://www.flowjo.com
Other
RPMI 1640 Medium	Thermo Fisher Scientific	Cat#A10491-01
Fetal Bovine Serum, Charcoal Stripped	Sigma	Cat#F6765
StemSpan SFEM II	STEMCELL Technologies	Cat#09605
StemSpan SFEM	STEMCELL Technologies	Cat#09650
RBC Lysis Solution	5 Prime	Cat#2301310
MethoCult	STEMCELL Technologies	Cat#M3534
Effectene	Qiagen	Cat#301425
Fugene 6 Transfection Reagent	Promega	Cat#E2691
Dynabeads® Protein G	Thermo Fisher Scientific	Cat#10004D
Dynabeads® Protein A	Thermo Fisher Scientific	Cat#10002D
Dynabeads™ MyOne™ Streptavidin C1	Thermo Fisher Scientific	Cat#65001
Agencourt AMPure XP 5 mL Kit	Beckman Coulter	Cat#A63880
Bio-Mag Plus Concanavalin A coated beads	Polysciences	Cat#86057
Protein A-MNase	Skene et al., 2015	N/A
Plamsmocin	Invivogen	Cat#ant-mpt
Retro-X Concentrator	Clontech	Cat#631455
Lenti-X Concentrator	Clontech	Cat#631231
Halt™ Protease Inhibitor Cocktail	Thermo Fisher Scientific	Cat#778429
Proteinase K (Fungal)	Thermo Fisher Scientific	Cat#25530-031
T4 polynucleotide kinase	New England Biolabs	Cat#M0201
Q5® High-Fidelity DNA Polymerase	New England Biolabs	Cat#M0491L
HindIII	New England Biolabs	Cat#R0104S
Dounce homogenizer, pestle A	Kimble Chase	Cat#885301-0002
Amicon® Ultra - 0.5ml-30K	Millipore	Cat#UFC5030BK
T4 DNA ligase	Thermo Fisher Scientific	Cat#15224
Taq DNA ligase and buffer	New England Biolabs	Cat#M0208S
Salmon testes DNA	Sigma	Cat#D7656
QIAquick gel extraction kit	Qiagen	Cat#28704
4-Thiouridine	Carbosynth	Cat#NT06186
EZ-Link™ HPDP-Biotin	Thermo Fisher Scientific	Cat#21341
T4 PNK (10 U/μL)	New England Biolabs	Cat#M0201S
T4 DNA polymerase	Thermo Fisher Scientific	Cat#18005025
Taq DNA polymerase	Thermo Fisher Scientific	Cat#EP0401
2× Rapid DNA ligase buffer	Enzymatics	Cat#B101L
Enzymatics DNA ligase	Enzymatics	Cat#L6030-HC-L
5× KAPA buffer	Kapabiosystems	Cat#KK2502
KAPA HS HIFI polymerase	Kapabiosystems	Cat#KK2502
Clean-Blot IP Detection Reagent	Thermo Fisher Scientific	Cat#21230