Table 1.
RNAi pathway components identified in the Psoroptes ovis genome
| Gene family (Organism) | Accession IDa | P. ovis gene IDb | Gene number | Top blast hit E-value |
|---|---|---|---|---|
| Small RNA biosynthesis | ||||
| Dicer (T. urticae) | tetur19g00520; tetur07g00990 | psovi14g04930; psovi280g04020; psovi298g00280 psovi14g04950; psovi280g04030; psovi14g04940 |
6 | 7e−117 |
| Drosha (T. urticae) | tetur12g00910 | psovi45g00290 | 1 | 0.0 |
| drh-1 (C. elegans) | F15B10.2 | psovi288g01850; psovi14g00590; psovi288g01290 | 3 | 2e−11 |
| Exportin-5 (T. urticae) | tetur02g00520; tetur02g00500 | psovi17g08260 | 1 | 2e−05 |
| Loquacious (T. urticae) | tetur13g00430; tetur13g00410 | psovi14g10890; psovi292g01850; psovi283g06140 | 3 | 1e−31 |
| Pasha (T. urticae) | tetur36g00220; tetur36g00250 | psovi05g03810 | 1 | 1e−161 |
| xpo-1 (C. elegans) | ZK742.1 | psovi36g04430 | 1 | 0.0 |
| xpo-2 (T. urticae) | tetur18g01350 | psovi09g00130; psovi52g00090 | 2 | 0.0 |
| RNA induced silencing complex (RISC) components | ||||
| ain-2 (C. elegans) | B0041.2 | psovi 298g00740 | 1 | 4e−04 |
| C3P0 (Tribolum castaneum) | ABX72055 | N/A | N/A | N/A |
| R2d2 (D. melanogaster) | NP_001285720.1 | N/A | N/A | N/A |
| tsn-1 (C. elegans) | F10G7.2 | psovi90g00040 | 1 | 4e−123 |
| vig-1 (T. urticae) | tetur22g01310 | psovi301g01900 | 1 | 9e−22 |
| RNAi inhibitors | ||||
| adr-2 (C. elegans) | T20H4.4 | psovi280g06440 | 1 | 9e−24 |
| eri-1 (C. elegans) | T07A9.5 | psovi17g06910 | 1 | 6e−28 |
| eri-6/7 (C. elegans) | C41D11.1 | psovi286g03350; psovi66g00290; psovi286g02110; psovi05g05100 | 4 | 9e−32 |
| xrn-1 (C. elegans) | Y39G8C.1 | psovi283g01010 | 1 | 0.0 |
| xrn-2 (T. urticae) | tetur25g00400 | psovi17g08800 | 1 | 0.0 |
| dsRNA uptake and spreading | ||||
| GW182 (T. urticae) | tetur05g07970; tetur09g00260 | psovi294g01730; psovi17g04680 | 2 | 1e−18 |
| rsd-3 (C. elegans) | C34E11.1 | psovi22g08140; psovi20g00980 | 2 | 4e−41 |
| Secondary amplification | ||||
| RdRP (T. urticae) | tetur02g08750; tetur02g08760; tetur02g08780; tetur02g08810 tetur02g08820 |
psovi20g00680; psovi69g00210; psovi14g08400 | 3 | 1e−61 |
| Nuclear effectors | ||||
| cid-1 (C. elegans) | psovi280g05740; psovi22g06910; psovi17g01850 psovi40g00030; psovi08g01430; psovi38g00730 psovi35g01570; psovi33g01410; psovi283g04640 psovi26g00430; psovi08g01710 |
11 | 3e−09 | |
| gfl-1 (C. elegans) | M04B2.3 | psovi14g08490; psovi283g02390 | 2 | 2e−41 |
| mes-2 (C. elegans) | R06A4.7 | psovi17g07450; psovi283g06100; psovi301g02970 psovi05g04620; psovi05g01520; psovi58g00680 |
6 | 3e−56 |
| mes-6 (C. elegans) | C09G4.5 | psovi280g00380; psovi14g10140 | 2 | 7e−12 |
| mut-2 (C. elegans) | K04F10.6 | psovi44g00310 | 1 | 2e−10 |
| mut-16 (C. elegans) | B0379.3 | psovi33g00580 | 1 | 5e−04 |
| rha-1 (C. elegans) | T07D4.3 | psovi45g00690; psovi14g03610; psovi22g06890 psovi26g00980; psovi17g03400; psovi22g00930 psovi43g00020; psovi14g10090; psovi298g00430 |
9 | 2e−128 |
| zfp-1 (C. elegans) | F54F2.2 | psovi26g03300; psovi17g07470; psovi09g00270; psovi294g01700; psovi09g02230 | 5 | 4e−54 |
| RISC—argonaute/piwi proteins | ||||
| Argonaute (T. urticae; D. farinae) | tetur20g02910; tetur09g03140 tetur09g00620; tetur02g10560 tetur02g10580; tetur04g01190 tetur02g10570; KY794591; KY794592 |
psovi295g00210 | 1 | 0.0 |
| D. farinae Divergent Ago Clade | KY794593; KY794594; KY794598; KY794596; KY794597; KY794598 | psovi280g02500; psovi280g02490; psovi26g02770; psovi294g02030; psovi302g00450 | 5 | 0.0 |
| P. ovis Divergent Ago Clade | N/A | psovi14g07720; psovi14g07690; psovi14g07730; psovi14g07700; psovi14g07710; psovi63g00040 | 6 | N/A |
| Piwi (T. urticae) | tetur06g03300 tetur28g00450; tetur28g00340 tetur06g05570; tetur06g05580 tetur06g05600; tetur17g03380 |
N/A | N/A | N/A |
| nrde-1 (T. urticae) | tetur21g02920 | psovi17g01230 | 1 | 5e−71 |
aTetranychus urticae accession numbers from Grbic et al. [47] (in format tetur00g00000).
bPsoroptes ovis accession numbers (in format psovi00g00000).