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. 2018 Nov 1;14(11):e1007653. doi: 10.1371/journal.pgen.1007653

Fig 2. BRC-1 is progressively enriched at the short arms of bivalents in a PLK-2-dependent manner.

Fig 2

(A) BRC-1::HA, HTP-3 and SYP-1 localization patterns at different stages of meiotic prophase I. TZ = transition zone, MP = mid-pachynema, LP = late pachynema, DP = diplonema. Scale bar, 5 μm. (B) BRC-1::HA co-staining with anti-PLK-2 and anti-SYP-1 shows BRC-1 recruitment concomitantly with PLK-2 and before SYP-1 to the shorts arm of bivalent. Insets of a representative nucleus from late pachytene/diplotene stage showing full co-localization of BRC-1::HA and PLK-2. Scale bar, 30 μm (left) and 5 μm (right). (C) Left: late pachytene-diplotene transition showing anti HA staining in controls and plk-2 mutants. Right: insets showing magnified nuclei from picture on the left, from mid-late pachytene (brown rectangle) and late pachytene-diplotene stages (green rectangle) stained for HA (BRC-1), SYP-1 and HTP-3. BRC-1::HA localization along SC and retraction towards the short arm of the bivalent are profoundly impaired by lack of plk-2 function.