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. 2018 Nov 1;14(11):e1007653. doi: 10.1371/journal.pgen.1007653

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© 2018 Janisiw et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) BRC-1::HA co-localizes with pro-CO factor COSA-1 in late prophase I. MP/LP = mid-/late pachynema, LP/DP = late pachynema/diplonema. Scale bar, 5 μm. (B) BRC-1::HA co-localizes with ZHP-3 and (C) GFP::MSH-5 in late pachytene nuclei. Scale bar, 5 μm. Bottom: Analysis of embryonic viability and segregation of male progeny shows full functionality of GFP::msh-5 and OLLAS::cosa-1 tagged lines. Embryos scored: WT (1311), GFP::msh-5 (1191), OLLAS::cosa-1 (1098), msh-5 (971), cosa-1 (927). (D) Partial projections of nuclei under super-resolution structured illumination microscopy: different examples show BRC-1::HA localization in the region surrounding the COSA-1-labeled CO site. BRC-1::HA forms a nodule-like structure together with SYP-1. (E) Insets showing magnified details from nuclei in (D).