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. 2018 Nov 1;14(11):e1007653. doi: 10.1371/journal.pgen.1007653

Fig 4. BRC-1 and BRD-1 form complexes with MSH-5 and SYP-3 in vivo.

Fig 4

(A) Pull-downs of GFP::MSH-5 and GFP::SYP-3 reveals presence in a complex with BRC-1::HA. (B) BRD-1::HA co-immunoprecipitates with GFP::MSH-5 and GFP::SYP-3 as well. (C) Presence in a protein complex of MSH-5 and BRC-1 was further validated by performing reciprocal IPs in which the GFP and HA tags were swapped (msh-5::2xHA and GFP::brc-1 strains). Non-specific binding of BRC-1 with GFP was ruled out by performing IPs of BRC-1::HA in a strain carrying an integrated GFP with ubiquitous expression (see Methods).