Figure 3.

Adam17 is a candidate for adaptive differences in body mass among mice in eastern North America. (A) Expression of Adam17 is correlated with latitude (P = 0.032, Pearson's correlation = −0.30). Sex was not a significant predictor of Adam17 expression. (B) A SNP at Chr 12: 21,332,631 was identified as a cis-eQTL for Adam17. (C) Allele frequencies of Chr 12: 21,332,631 in five populations. (D) The LFMM |z-scores| for sites on Chromosome 12 versus position. Points above the red line were considered clinal outliers in this study. The red box represents the peak in which Chr 12: 21,332,631 is found. (E) Nearby outlier SNPs in LD with Chr 12: 21,332,631. Correlations (r², %) are given in each block. The z-scores for each site's association with latitude are given in parentheses. (F) Adam17 expression is significantly associated with body mass when controlling for latitude (Pearson's correlation, P = 4.6 × 10⁻⁴, R² = 0.22). (G) Genotype at Chr 12: 21,332,631, the cis-eQTL for Adam17, significantly trends with body size when latitude is controlled for (Cochran-Armitage trend test, P = 0.034).