| Demographics (age, sex, weight, ethnicity) |
Needle gauge |
Time to storage |
Storage temperature |
| Diet, including supplements |
Needle composition |
Centrifugation (presence and type of separator,
temperature, number of rounds [single or double]) |
Storage volume |
| Health status |
Withdrawal site |
Addition of protease inhibitor |
Number of freeze-thaw cycles |
| Medication |
Collection tube characteristics |
Use of denaturation step or protein extraction |
Duration |
| Drug/alcohol use |
Anticoagulant use |
Use of plasma or serum |
|
| Medical conditions |
Time of collection |
|
|
| Exercise |
|
|
|
| Posture/bed rest |
|
|
|
