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(A) Plot of Btotal(τ) (gray), c(τ) (red), and Bfree(τ) (obscured by c(τ)). ki = k−i = 1x10-8 s-1, Λ = 83,333 hr, and L0 = Kd = 1 nM, which yields 50% SSO at all times (denoted by equal lengths of the vertical arrows above and below their respective centroids), irrespective of kon and koff (holding Kd constant at 1 nM). (B) Same as A, except with L0 = 19 ⋅ Kd, which yields the expected SSO = 95%.
