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. 2018 Nov 1;13(11):e0206748. doi: 10.1371/journal.pone.0206748

Fig 1. PEITC inhibits PTP1B with concomitant decrease in cysteinyl thiol.

Fig 1

(A) The structure of PEITC. (B) Concentration-dependent inhibition of PTP1B by PEITC. Recombinant PTP1B was incubated with the indicated concentration of PEITC in HEPES buffer (pH 7.5) at 37°C for 30 min, and then PTP1B activity was measured. (C) A proposed mechanism for the inhibition of PTP1B through the reaction of ITC with the cysteinyl thiol. (D) Concentration-dependent modification of cysteinyl thiol in PTP1B by PEITC. Recombinant PTP1B was incubated with the indicated concentration of PEITC in HEPES buffer (pH 7.5) at 37°C for 30 min. Then, the reactive thiol group and total PTP1B were determined by biotin-labeling assay and immunoblotting, respectively. Data are representative of two independent experiments.