Table 2. Primer sequences used for qRT-PCR.
gene | forward primer 5’ to 3’ | reverse primer 5’ to 3’ |
---|---|---|
NCAM-120 | GAACCTGATCAAGCAGGATGACGG | CTAACAGAGCAAAAGAAGAGTC |
NCAM-140 | GTCCTGCTCCTGGTGGTTGTG | CCTTCTCGGGCTCCGTCAGT |
NCAM-180 | CGAGGCTGCCTCCGTCAGCACC | CCGGATCCATCATGCTTTGCTCTC |
FGFR1 | GGCTACAAGGTCCGTTATGCC | GATGCTGCCGTACTCATTCTC |
S100A4 | TCTTTCTTGGTTTGATCCTG | GCATCAAGCACGTGTCTGAA |
ACTA2 | AAGCACAGAGCAAAAGAGGAAT | ATGTCGTCCCAGTTGGTGAT |
SNAI2 | ACTCCGAAGCCAAATGACAA | CTCTCTCTGTGGGTGTGTGT |
SNAI1 | GGCAATTTAACAATGTCTGAAAAGG | GAATAGTTCTGGGAGACACATCG |
TWIST1 | CTCAAGAGGTCGTGCCAATC | CCCAGTATTTTTATTTCTAAAGGTGTT |
MMP2 | TACAGGATCATTGGCTACACACC | GGTCACATCGCTCCAGACT |
MMP9 | TGTACCGCTATGGTTACACTCG | GGCAGGGACAGTTGCTTCT |
ITGA5 | GGCTTCAACTTAGACGCGGAG | TGGCTGGTATTAGCCTTGGGT |
ITGB1 | GTAACCAACCGTAGCAAAGGA | TCCCCTGATCTTAATCGCAAAAC |
CDH1 | CATGAGTGTCCCCCGGTATC | CAGTATCAGCCGCTTTCAGA |
CDH2 | TCAGGCGTCTGTAGAGGCTT | ATGCACATCCTTCGATAAGACTG |