(A) Quantification of E/A fraction in wild-type (n = 10) and miR-29a/b1−/− (n = 9) mice (two-tailed Student t test with Welch’s correction). (B) Quantification of DT of early filling in wild-type (n = 10) and miR-29a/b1−/− (n = 9) mice. (C) Quantification of IVRT in wild-type (n = 10) and miR-29a/b1−/− (n = 9) mice. (D) Ratio of left lung weight to body weight in wild-type (n = 8) and miR-29a/b1−/− (n = 5) mice (two-tailed Student t test with Welch’s correction). (E) HE and Masson’s trichrome stained lung sections of wild-type and miR-29a/b1−/− mice (original magnification: ×4, scale bar: 500 μm). (F) Clinical score of pulmonary congestion in wild-type (n = 15) and miR-29a/b1−/− (n = 20) mice. Original raw data can be found in S1 Data file. DT, deceleration time; E/A, early and late diastolic filling velocities ratio; HE, hematoxylin–eosin; HFpEF, heart failure with preserved ejection fraction; IVRT, isovolumetric relaxation time; NS, non-significant; TRI, Masson’s trichrome; WT, wild-type.