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. 2018 Oct 22;16(10):e2006247. doi: 10.1371/journal.pbio.2006247

Fig 5. Systemic hypertension and vascular remodeling in miR-29a/b1−/− mice.

Fig 5

(A) Systolic, (B) diastolic, and (C) mean blood pressure values from wild-type (n = 10) and miR-29a/b1−/− (n = 8) mice. (D) Hematoxylin–eosin (HE), orcein for elastic fibers (Orcein), von Willebrand factor (VWF), and α-smooth muscle actin (SMA) staining of small pulmonary lung vessels (<50 μm) of wild-type and miR-29a/b1−/− mice (original magnification: ×40, scale bars: 50 μm). (E) Quantification of media layer thickness/diameter ratio of five SMA-stained small pulmonary blood vessels per mouse in wild-type (n = 4) and miR-29a/b1−/− (n = 4) mice. (F) Micrographs of Verhoeff–Van Gieson elastic staining of aortas from representative wild-type and miR-29a/b1−/− mice (original magnification: ×10, scale bar: 50 μm). (G) Thickness of the aortic adventitia layer of wild-type (n = 10) and miR-29a/b1−/− mice (n = 11). (H) Thickness of the aortic media layer of wild-type (n = 9) and miR-29a/b1−/− (n = 11) mice. (I) Representative micrographs of coronary arteries stained with Masson’s trichrome of wild-type and miR-29a/b1−/− mice (original magnification: ×20, scale bars: 100 μm). (J) Quantification of the surrounding fibrotic area/perimeter ratio of six coronary arteries per mouse in wild-type (n = 4) and miR-29a/b1−/− (n = 9) mice. Original raw data can be found in S1 Data file. Diast., diastolic; HE, hematoxylin–eosin; Orcein, orcein for elastic fibers; pres., pressure; SMA, α-smooth muscle actin; Syst., systolic; VWF, von Willebrand factor; WT, wild-type.