Table 1. Primer pairs used for identification and sequencing of vip3 gene in the Bt isolates.
Primers | Sequencea (5’-3’) | Nucleotide position in the reference sequence | Amplicon size (bp) | References | |
---|---|---|---|---|---|
Screening primers | vip3-sc(f) | TGCCACTGGTATCAARGA | - | - | [10] |
vip3-scII(r) | CCATTAATYGGAKTCAAAAATGTTTCACTGAT | 78–1472 | 1395 | [11] | |
Typing primers | vip3A-F1 | ASTTTAAGATATGAGGYAACAGC | - | - | In this work |
vip3Aa-R1 | CATCGTAAAAATGTACAATAGGA | 1297–2356 | 1060 | In this work | |
vip3Af-R1 | TCAAATGATATATGACCACCA | 1297–2348 | 1052 | In this work | |
vip3Ag-R1 | ATGTAAAACGAGAAAGCTCTACA | 1297–2323 | 1027 | In this work |
a Universal code for degenerate bases: R = A, G; Y = C, T; K = G,T; S = G, C.