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. 2018 Nov 1;13(11):e0206813. doi: 10.1371/journal.pone.0206813

Table 1. Primer pairs used for identification and sequencing of vip3 gene in the Bt isolates.

Primers Sequencea (5’-3’) Nucleotide position in the reference sequence Amplicon size (bp) References
Screening primers vip3-sc(f) TGCCACTGGTATCAARGA - - [10]
vip3-scII(r) CCATTAATYGGAKTCAAAAATGTTTCACTGAT 78–1472 1395 [11]
Typing primers vip3A-F1 ASTTTAAGATATGAGGYAACAGC - - In this work
vip3Aa-R1 CATCGTAAAAATGTACAATAGGA 1297–2356 1060 In this work
vip3Af-R1 TCAAATGATATATGACCACCA 1297–2348 1052 In this work
vip3Ag-R1 ATGTAAAACGAGAAAGCTCTACA 1297–2323 1027 In this work

a Universal code for degenerate bases: R = A, G; Y = C, T; K = G,T; S = G, C.