Table 5. Protein and peptide profiles of Bt isolates as obtained by LC-MS/MS.
|
Protein |
Number of peptides with contribution value > 2 | |||||
|---|---|---|---|---|---|---|
| Isolate | Protein name a | Unused ProtScore | Sequence coverage (%) | No. of peptides | Shared peptidesb | Unique peptidesc |
| 6A | Cry1Ac | 568.03 | 75.81 | 611 | 215 | 3 |
| Cry2A | 92.95 | 65.09 | 71 | |||
| 42MY | Cry1Ac | 572.39 | 64.43 | 449 | 232 | 2 |
| Cry1Ea | 180.36 | 69.09 | 372 | 66 | 6 | |
| Cry2A | 20.86 | 36.02 | 24 | |||
| 45MY | Cry1Ac | 392.57 | 78.78 | 532 | 120 | 3 |
| Cry1Ea | 138.46 | 75.06 | 343 | 40 | 7 | |
| Cry2A | 274.67 | 92.58 | 352 | |||
| 51MY | Cry1Ac | 452.21 | 79.63 | 606 | 161 | 8 |
| Cry1Ea | 195.91 | 77.54 | 440 | 60 | 6 | |
| Cry2A | 151.04 | 81.99 | 171 | |||
| Bt-KH58 | Cry1Aa | 594.3 | 57.57 | 551 | 232 | 2 |
| Cry1Ac | 83.06 | 60.61 | 566 | 29 | 3 | |
| Cry2A | 51.17 | 42.97 | 39 | |||
a The accession number of the proteins in the database are: Cry1Aa, P0A368; Cry1Ac, P05068; Cry1Ea, Q57458.
b Number of peptides with a contribution value higher than 2 that are present in two or more proteins of the Cry1 or Cry2 protein families.
c Number of peptides with a contribution value higher than 2 that are present only once in one protein of the Cry family.