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. Author manuscript; available in PMC: 2019 Oct 10.
Published in final edited form as: Neuron. 2018 Oct 10;100(1):75–90.e5. doi: 10.1016/j.neuron.2018.09.014

Figure 4.

Figure 4

3K αS accumulates at vesicles and forms proteinase K resistant, phosphorylated, and truncated αS deposits mouse brain. (a, b) Hu (15G7) αS IHC (age 6 mo) without (a) and with (b) proteinase K pre-treatment. (c) IHC with abs to ser129-phosphorylated (phos) and ΔC-truncated (105) αS. (d) Immunogold EM analysis of 6 mo brains with 15G7 (bottom row: high power). (e) Quant. of αS immunogold particles at synaptic vesicles. 10 fields each from n=2 brains per genotype run in 2 ind. exps. (blinded counting). Means ± SEM. *p<0.05, **p<0.01, unpaired two-tailed Student’s t test. Scale bars (a-c) 20 µm, (d) 60 nm.