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. 2018 Oct 16;7:e39655. doi: 10.7554/eLife.39655

Figure 2. WDR34 and WDR60 are essential for IFT-B trafficking in primary cilia.

Figure 2.

(A) Localization of IFT88 in WT, WDR60 KO, and WDR34 KO#1 cells stained with the ciliary marker Acetylated tubulin. (Ai) Quantification of IFT88 localization within cilia in WT and WDR60 KO cells (102 WT and 101 WDR60 cells quantified). (B and C) Localization of IFT88 (B) and IFT54 (C) in WT, WDR60 KO, and WDR34 KO#1 cells stained with the centrosome marker gamma-tubulin. (D) Localization of IFT57 in WT, WDR60 KO, and WDR34 KO#1 cells stained with acetylated tubulin. (Di) Quantification of IFT57 within cilia in WDR60 KO and WT cells (IFT57 106 WT and 98 WDR60 KO cells quantified; n = 3 independent experiments). Mann-Whitney test was used, p-value: ****=<0.0001. (E) Endogenous IFT20 accumulates at the ciliary tip in WDR60 KO cells. (F) Quantification of IFT20 localization within cilia in WDR60 KO cells (n = 3 independent experiments, 102 WT and 100 WDR60 KO cells quantified). (G) Localization of IFT20-GFP in WT and WDR60 KO fixed cells. Scale bar, all panels = 5 µm. Arrows point to the ciliary base.