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(A–C) Localization of (A) RPGRIP1L, (B) TMEM67, and (C) TCTN1 in WT and KO cells. Enlargements from the box regions are shown on the right. (Ai and Bi) Percentage of RPGRIP1L and TMEM67 positive cilia (RPGRIP1L n = 3, 188 WT, and 272 WDR60 KO cells quantified; TMEM67 n = 3, 359 WT, and 243 WDR60 KO cells quantified). Mann-Whitney test was used p-value: ****=<0.0001. Scale bars 5 μm. Arrows point to the ciliary base.
