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. 2018 Oct 16;7:e39655. doi: 10.7554/eLife.39655

Author response image 1. (A) RNAi of WDR34 and WDR60 validated by immunoblotting with GAPODH as a loading control.

Author response image 1.

(B) Immunofluorescence of TMEM67 and RPGRIP1L in WDR34 and WDR60 depleted cells. TMEM67 is seen at the base and within the cilium proximal to this in both control and depleted cells, RPGRIP1L is more tightly restricted to the base of the cilium in all cases. These are representative images, we have analysed multiple fields of view in each case using z-stacks and cannot identify any difference.