Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Kcnq2 | NA | NCBI_Gene:16536; MGI:1309503 |
|
| Gene (Mus musculus) | Kcnq3 | NA | NCBI_Gene:110862; MGI:1336181 |
|
| Strain, strain background (M. musculus, Emx1IRESCre, C57BL/6J background) |
B6.129S2-Emx1tm1(cre)Krj /J | PMID: 12151506 | RRID:IMSR_JAX:005628 | |
| Strain, strain background (M. musculus, Nkx2.1-Cre, C57BL/6J background) |
C57BL/6J-Tg (Nkx2-1-cre)2Sand/J |
PMID: 17990269 | RRID:IMSR_JAX:008661 | |
| Strain, strain background (M. musculus, Sst-IRES-Cre, C57BL/6J background) |
Ssttm2.1(cre)Zjh/J | PMID: 21943598 | RRID:IMSR_JAX:013044 | |
| Strain, strain background (M. musculus, Pvalb-Cre, C57BL/6J background) |
B6;129P2-Pvalbtm1(cre)Arbr/J | PMID: 15836427 | RRID:IMSR_JAX:008069 | |
| Strain, strain background (M. musculus, tdTomato reporter Ai9, C57BL/6J background) |
B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J |
PMID: 20023653 | RRID:IMSR_JAX:007909 | |
| Strain, strain background (M. musculus, Kcnq2f/f, C57BL/6J background) |
Kcnq2f/f | PMID: 24719109 | N-A | |
| Strain, strain background (M. musculus, Kcnq3f/f, C57BL/6J background) |
Kcnq3f/f | PMID: 24719109 | N-A | |
| Antibody | Alexa fluor 488 streptavidin |
Invitrogen | Invitrogen:S32354; RRID:AB_2315383 |
(1:500) |
| Antibody | anti-Lucifer yellow (rabbit polyclonal) |
Invitrogen | Invitrogen:A5750; RRID:AB_2536190 |
(1:500) |
| Antibody | anti-Somatostatin (rat monoclonal) |
Millipore | Millipore:MAB354; RRID:AB_2255365 |
(1:250) |
| Antibody | Alexa fluor 488 anti-rabbit secondary (goat polyclonal) |
Invitrogen | Invitrogen:A11034; RRID:AB_2576217 |
(1:500) |
| Antibody | Alexa fluor 647 anti-rat secondary (goat polyclonal) |
Invitrogen | Invitrogen:A21247; RRID:AB_141778 |
(1:500) |
| Sequence-based reagent |
somatostatin mRNA probe (mouse); Mm-Sst-C1 |
ACDBio | Cat#:404631 | (1:50) |
| Sequence-based reagent |
parvalbumin mRNA probe (mouse); Mm-Pvalb-C1 |
ACDBio | Cat#:421931 | (1:50) |
| Sequence-based reagent |
tdTomato mRNA probe (mouse); Mm-tdTomato-C3 |
ACDBio | Cat#:317041-C3 | (1:50) |
| Sequence-based reagent |
Kcnq2 mRNA probe (mouse); Mm-Kcnq2-O1 |
ACDBio; this paper | Cat#:300031-C2 | (1:50); custom made probe that targets exons 2–5 of Kcnq2 |
| Sequence-based reagent |
Kcnq3 mRNA probe (mouse); Mm-Kcnq3-O1 |
ACDBio; this paper | Cat#:300031-C3 | (1:50); custom made probe that targets exons 2–5 of Kcnq2 |
| Commercial assay or kit |
RNAscope Fresh Frozen Multiplex Fluorescent kit |
ACDBio | Cat#:320851 | |
| Chemical compound, drug |
CNQX | Abcam | ab120017 | |
| Chemical compound, drug |
D-AP5 | Abcam | ab120003 | |
| Chemical compound, drug |
Picrotoxin | Abcam | ab120315 | |
| Chemical compound, drug |
Tetrodotoxin; TTX | Abcam | ab120054 | |
| Chemical compound, drug |
XE-991 | Abcam | ab120089 | |
| Chemical compound, drug |
Lucifer Yellow | Sigma | Cat#:B4261 | (0.1%) |
| Chemical compound, drug |
Biocytin | Molecular Probes | Cat#:L1177 | (0.05%) |
| Software, algorithm | Prism 7 | GraphPad | RRID:SCR_002798 | Version 7.03 |
| Software, algorithm | Clampfit 10 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | Minianalysis | Synaptosoft | RRID:SCR_002184 | |
| Software, algorithm | Origin 8 Pro | OriginLab | RRID:SCR_014212 | Version 8.0951 |
| Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | Version 2.0.0 |