Table 2.
Post-translational modifications in PTB proteins under splicing reaction conditions | ||||||
---|---|---|---|---|---|---|
PTBP1 | PTBP2 | |||||
Residue No. | % Acetylation | % Phosphorylation | Residue No. | % Acetylation | % Phosphorylation | |
N-terminal region | S16 | ┄ | 1.50 ±0.20 | S16 | ┄ | 1.40 ±0.40 |
S21 | ┄ | 0.85 ±0.10 | S21 | ┄ | 3.80 ± 1.30 | |
T22 | ┄ | 0.64 ± 0.22 | G22 | ┄ | ┄ | |
N26 | ┄ | ┄ | S26 | ┄ | 2.10 ± 1.00 | |
G27 | ┄ | ┄ | S27 | ┄ | 1.92 ±1.19 | |
I30 | ┄ | ┄ | S30 | ┄ | 1.08 ±0.33 | |
S33 | ┄ | 1.13 ±0.68 | S33 | ┄ | 0.74 ± 0.04 | |
N34 | ┄ | ┄ | S34 | ┄ | 1.44 ±0.66 | |
S35 | ┄ | 0.72 ± 0.24 | M35 | ┄ | ┄ | |
A38 | ┄ | ┄ | T38 | ┄ | 1.10 ± 0.31 | |
S44 | ┄ | ┄ | S44 | ┄ | 0.75 ± 0.35 | |
K45 | ┄ | ┄ | K45 | 7.55 ±4.35 | ┄ | |
K46 | ┄ | ┄ | K46 | 1.22 ±0.68 | ┄ | |
RRM1 | K92 | 7.65 ±4.15 | ┄ | K92 | 8.10 ±5.20 | ┄ |
K94 | 4.30 ± 1.60 | ┄ | K94 | 11.20 ±8.80 | ┄ | |
Linker-1 | N155 | ┄ | ┄ | T154 | ┄ | 7.45 ± 2.05 |
S156 | ┄ | 5.60 ± 1.20 | A155 | ┄ | ┄ | |
S159 | ┄ | 1.75 ±0.35 | T158 | ┄ | 3.55 ± 0.95 | |
L162 | ┄ | ┄ | T161 | ┄ | 2.25 ± 0.05 | |
A165 | ┄ | ┄ | S164 | ┄ | 5.55 ± 1.25 | |
S167 | ┄ | 4.35 ± 0.55 | ||||
A169 | ┄ | ┄ | T166 | ┄ | 5.60 ± 0.80 | |
A170 | ┄ | ┄ | T167 | ┄ | 4.35 ±2.05 | |
G174 | ┄ | ┄ | S171 | ┄ | 3.15 ±0.95 | |
RRM2 | K218 | 4.50 ± 3.20 | ┄ | K215 | ┄ | ┄ |
K259 | 12.00 ±8.00 | ┄ | K256 | 11.50 ±8.50 | ┄ | |
K266 | 9.65 ± 8.55 | ┄ | K263 | 4.15 ±2.55 | ┄ | |
Linker-2 | L325 | ┄ | ┄ | T298 | ┄ | 26.50 ± 13.50 |
A335 | ┄ | ┄ | S308 | ┄ | 15.00 ± 1.70 | |
S341 | ┄ | 2.15 ±0.95 | A316 | ┄ | ┄ | |
RRM3 | K394 | 28.55 ±21.54 | ┄ | K369 | ┄ | ┄ |
K400 | 5.23 ± 4.27 | ┄ | K375 | 4.00 ± 0.40 | ┄ | |
K424 | 11.70 ±6.20 | ┄ | K399 | 11.05 ±8.05 | ┄ | |
K436 | 15.90 ±9.10 | ┄ | K411 | 15.45 ±8.55 | ┄ | |
T453 | ┄ | ┄ | T428 | ┄ | 10.80 ±5.90 | |
K454 | 15.45 ± 13.15 | ┄ | K429 | ┄ | ┄ | |
RRM4 | K508 | 6.85 ±0.25 | ┄ | K483 | 8.50 ± 5.80 | ┄ |
The data reported are the average of two independent experiments.