Table 2.

Post-Translational Acetate and Phosphate Modifications in PTBPs Incubated in Splicing Reaction Mixtures Containing HeLa Cell Nuclear Extract^a

Post-translational modifications in PTB proteins under splicing reaction conditions
	PTBP1			PTBP2
	Residue No.	% Acetylation	% Phosphorylation	Residue No.	% Acetylation	% Phosphorylation
N-terminal region	S16	┄	1.50 ±0.20	S16	┄	1.40 ±0.40
	S21	┄	0.85 ±0.10	S21	┄	3.80 ± 1.30
	T22	┄	0.64 ± 0.22	G22	┄	┄
	N26	┄	┄	S26	┄	2.10 ± 1.00
	G27	┄	┄	S27	┄	1.92 ±1.19
	I30	┄	┄	S30	┄	1.08 ±0.33
	S33	┄	1.13 ±0.68	S33	┄	0.74 ± 0.04
	N34	┄	┄	S34	┄	1.44 ±0.66
	S35	┄	0.72 ± 0.24	M35	┄	┄
	A38	┄	┄	T38	┄	1.10 ± 0.31
	S44	┄	┄	S44	┄	0.75 ± 0.35
	K45	┄	┄	K45	7.55 ±4.35	┄
	K46	┄	┄	K46	1.22 ±0.68	┄
RRM1	K92	7.65 ±4.15	┄	K92	8.10 ±5.20	┄
	K94	4.30 ± 1.60	┄	K94	11.20 ±8.80	┄
Linker-1	N155	┄	┄	T154	┄	7.45 ± 2.05
	S156	┄	5.60 ± 1.20	A155	┄	┄
	S159	┄	1.75 ±0.35	T158	┄	3.55 ± 0.95
	L162	┄	┄	T161	┄	2.25 ± 0.05
	A165	┄	┄	S164	┄	5.55 ± 1.25
	S167	┄	4.35 ± 0.55
	A169	┄	┄	T166	┄	5.60 ± 0.80
	A170	┄	┄	T167	┄	4.35 ±2.05
	G174	┄	┄	S171	┄	3.15 ±0.95
RRM2	K218	4.50 ± 3.20	┄	K215	┄	┄
	K259	12.00 ±8.00	┄	K256	11.50 ±8.50	┄
	K266	9.65 ± 8.55	┄	K263	4.15 ±2.55	┄
Linker-2	L325	┄	┄	T298	┄	26.50 ± 13.50
	A335	┄	┄	S308	┄	15.00 ± 1.70
	S341	┄	2.15 ±0.95	A316	┄	┄
RRM3	K394	28.55 ±21.54	┄	K369	┄	┄
	K400	5.23 ± 4.27	┄	K375	4.00 ± 0.40	┄
	K424	11.70 ±6.20	┄	K399	11.05 ±8.05	┄
	K436	15.90 ±9.10	┄	K411	15.45 ±8.55	┄
	T453	┄	┄	T428	┄	10.80 ±5.90
	K454	15.45 ± 13.15	┄	K429	┄	┄
RRM4	K508	6.85 ±0.25	┄	K483	8.50 ± 5.80	┄

^aThe data reported are the average of two independent experiments.