Figure 1. Distribution of C57/BL6 Mouse Nuclear Receptor mRNA.

(A) The number of NRs expressed in various tissues is indicated in the pie chart and their names are shown in the table on the right. NRs were grouped into three categories based on their tissue distribution: Restricted (expressed in less than 50% of tissues), Widespread (expressed in more than 50% but not all tissues), and In All Tissues (expressed in 100% of tissues).

(B) The levels of NR expression in different tissue systems are indicated by the pie charts and their names are shown in the tables to the right. Normalized NR mRNA-expression levels were defined as Absent if the Ct value was ≥34, Low if the level was below 0.1 arbitrary units, Moderate if the level was between 0.1 and 1, and High if the level was >1.0 arbitrary units. Tissues systems were categorized as follows: central nervous system (eye, brainstem, cerebellum, cerebrum, corpus striatum, olfactory bulb, spinal cord, hypothalamus, and pituitary); nonreproductive endocrine system (adrenal, thyroid, and pancreas); gastroenteric system (tongue, stomach, duodenum, jejunum, ileum, colon, and gall bladder); metabolic system (liver, kidney, brown and white adipose, and muscle); immune system (spleen and thymus); reproductive system (ovary, uterus, epididymus, preputial gland, prostate, seminal vesicles, testis, and vas deferens); cardiovascular system (aorta, heart, and lungs); and structural system (bone and skin). Note that tissues were pooled from male mice (n = 6) except for ovary and uterus. An asterisk (*) indicates NRs that have a strong circadian expression and were lowest at time of analysis (see accompanying manuscript by Yang et al., 2006 in this issue of Cell).